The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.

Interferon-Resistant Daudi Cells

Inducible Expression of Human Gamma Interferon

Background:The premature termination of high producer clones, which will be killed due to cell proliferation and proteins production antagonism, is one of the basic drawback in recombinant proteins technology. Furtheremore, it is supposed some toxic proteins like interferon which we intended to clone and express, inhibit host cells’ proliferation. So, it is necessary to tightly control IFN-γ pr...

Interferon - a Down - regulates Insulin Receptors in Lymphoblastoid ( Daudi ) Cells

The Daudi line of human lymphoblastoid cells requires insulin and transferrin for growth in serumfree medium and is highly sensitive to the inhibitory effect of human leukocyte interferon (IFN-a) on cell proliferation. A variant subline of Daudi cells, which is resistant to the antiproliferative action of IFN-a, also has been grown in serum-free medium containing insulin and transferrin. The pr...

Interferon-induced gene expression in wild-type and interferon-resistant human lymphoblastoid (Daudi) cells.

Interferon-induced gene expression was analyzed in wild-type and interferon-resistant Daudi cells. Two classes of alpha-interferon-induced mRNAs and proteins were observed: those that were similarly induced in both types of cell and those that were induced only in the wild-type cells. Furthermore, the level of c-myc mRNA decreased in the wild-type but not in the resistant cells. This differenti...

Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells.

The recent observation made in our laboratory that cellular myc (c-myc) mRNA has a very short half-life in a variety of normal and transformed human cells emphasized the potential importance of post-transcriptional regulation of c-myc gene expression. Jonak and Knight [Jonak, G. J. & Knight, E., Jr. (1984) Proc. Natl. Acad. Sci. USA 81, 1747-1750] have reported a selective reduction of c-myc mR...